ePrints

Quantifying Missing (Phospho)Proteome Regions with the Broad-Specificity Protease Subtilisin

Lookup NU author(s): Dr Andreas Roos

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

© 2017 American Chemical Society.Despite huge efforts to map the human proteome using mass spectrometry the overall sequence coverage achieved to date is still below 50%. Reasons for missing areas of the proteome comprise protease-resistant domains including the lack/excess of enzymatic cleavage sites, nonunique peptide sequences, impaired peptide ionization/separation and low expression levels. To access novel areas of the proteome the beneficial use of enzymes complementary to trypsin, such as Glu-C, Asp-N, Lys-N, Arg-C, LysargiNase has been reported. Here, we present how the broad-specificity protease subtilisin enables mapping of previously hidden areas of the proteome. We systematically evaluated its digestion efficiency and reproducibility and compared it to the gold standard in the field, trypsin. Notably, subtilisin allows reproducible near-complete digestion of cells lysates in 1-5 min. As expected from its broad specificity the generation of overlapping peptide sequences reduces the number of identified proteins compared to trypsin (8363 vs 6807; 1% protein FDR). However, subtilisin considerably improved the coverage of missing and particularly proline-rich areas of the proteome. Along 14:628 high confidence phosphorylation sites identified in total, only 33% were shared between both enzymes, while 37% were exclusive to subtilisin. Notably, 926 of these were not even accessible by additional in silico digestion with either Asp-N, Arg-C, Glu-C, Lys-C, or Lys-N. Thus, subtilisin might be particularly beneficial for system-wide profiling of post-translational modification sites. Finally, we demonstrate that subtilisin can be used for reporter-ion based in-depth quantification, providing a precision comparable to trypsin - despite broad specificity and fast digestion that may increase technical variance.

Publication metadata

Author(s): Gonczarowska-Jorge H, Loroch S, Dell'Aica M, Sickmann A, Roos A, Zahedi RP

Publication type: Article

Publication status: Published

Journal: Analytical Chemistry

Year: 2017

Volume: 89

Issue: 24

Pages: 13137-13145

Print publication date: 19/12/2017

Online publication date: 14/11/2017

Acceptance date: 13/11/2017

ISSN (print): 0003-2700

ISSN (electronic): 1520-6882

Publisher: American Chemical Society

URL: https://doi.org/10.1021/acs.analchem.7b02395

DOI: 10.1021/acs.analchem.7b02395

Altmetrics

Share