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Electrochemical detection of plasma immunoglobulin as a biomarker for Alzheimer’s disease

Lookup NU author(s): Dr William Garyfallou, Orlando Ketebu, Samet Sahin, Dr Elizabeta Mukaetova-Ladinska, Professor Mike Catt, Professor Eileen Yu

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This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Abstract

© 2017 by the authors. Licensee MDPI, Basel, Switzerland. The clinical diagnosis and treatment of Alzheimer’s disease (AD) represent a challenge to clinicians due to the variability of clinical symptomatology as well as the unavailability of reliable diagnostic tests. In this study, the development of a novel electrochemical assay and its potential to detect peripheral blood biomarkers to diagnose AD using plasma immunoglobulins is investigated. The immunosensor employs a gold electrode as the immobilizing substrate, albumin depleted plasma immunoglobulin as the biomarker, and polyclonal rabbit Anti-human immunoglobulin (against IgA, IgG, IgM) as the receptor for plasma conjugation. The assay showed good response, sensitivity and reproducibility in differentiating plasma immunoglobulin from AD and control subjects down to 10− 9 dilutions of plasma immunoglobulin representing plasma content concentrations in the pg mL− 1 range. The newly developed assay is highly sensitive, less time consuming, easy to handle, can be easily modified to detect other dementia-related biomarkers in blood samples, and can be easily integrated into portable devices.

Publication metadata

Author(s): Garyfallou G-Z, Ketebu O, Sahin S, Mukaetova-Ladinska EB, Catt M, Yu EH

Publication type: Article

Publication status: Published

Journal: Sensors

Year: 2017

Volume: 17

Issue: 11

Online publication date: 27/10/2017

Acceptance date: 20/10/2017

Date deposited: 13/11/2017

ISSN (electronic): 1424-8220

Publisher: MDPI AG

URL: https://doi.org/10.3390/s17112464

DOI: 10.3390/s17112464

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