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Lookup NU author(s): Dr Yanny Baba Ismail, Dr Ana Ferreira-DuarteORCiD, Dr Oana Bretcanu, Professor Kenneth Dalgarno
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
This paper presents a new approach in assembling bone extracellular matrix components onto PLA films, and investigates the most favourable environment which can be created using the technique for cell-material interactions. Poly (lactic acid) (PLA) films were chemically modified by covalently binding the poly(ethylene imine) (PEI) as to prepare the substrate for immobilization of polyelectrolyte multilayers (PEMs) coating. Negatively charged polyelectrolyte consists of well-dispersed silicon-carbonated hydroxyapatite (SiCHA) nanopowders in hyaluronic acid (Hya) was deposited onto the modified PLA films followed by SiCHA in collagen type I as the positively charged polyelectrolyte. The outermost layer was finally cross-linked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrocholoride and N-hydroxysulfosuccinimide sodium salt (EDC/NHS) solutions. The physicochemical features of the coated PLA films were monitored via X-ray Photoelectron Spectroscopy (XPS) and Atomic Force Microscope (AFM). The amounts of calcium and collagen deposited on the surface were qualitatively and quantitatively determined. The surface characterizations suggested that 5-BL has the optimum surface roughness and highest amounts of calcium and collagen depositions among tested films. In vitro human mesenchymal stem cells (hMSCs) cultured on the coated PLA films confirmed that the coating materials greatly improved cell attachment and survival compared to unmodified PLA films. The cell viability, cell proliferation and Alkaline Phosphatase (ALP) expression on 5-BL were found to be the most favourable of the tested films. Hence, this newly developed coating materials assembly could contribute to the improvement of the bioactivity of polymeric materials and structures aimed to bone tissue engineering applications.
Author(s): Baba Ismail YM, Ferreira AM, Bretcanu O, Dalgarno K, El Haj AJ
Publication type: Article
Publication status: Published
Journal: Colloids and Surfaces B: Biointerfaces
Year: 2017
Volume: 159
Pages: 445-453
Print publication date: 01/11/2017
Online publication date: 14/08/2017
Acceptance date: 25/07/2017
Date deposited: 17/04/2018
ISSN (print): 0927-7765
ISSN (electronic): 1873-4367
Publisher: Elsevier
URL: https://doi.org/10.1016/j.colsurfb.2017.07.086
DOI: 10.1016/j.colsurfb.2017.07.086
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