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Loop-mediated isothermal amplification for rapid detection of the causal agents of cassava brown streak disease

Lookup NU author(s): Professor Neil Boonham

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Abstract

The causal agents of cassava brown streak disease have recently been identified as Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Primers have been developed for rapid detection of these viruses by reverse transcription loop-mediated isothermal amplification (RT-LAMP). Performance of the RT-LAMP assays compared favourably with published RT-PCR and real-time RT-PCR methods. Furthermore, amplification by RT-LAMP is completed in 40. min and does not require thermal cycling equipment. Modification of the RT-LAMP reactions to use labelled primers allowed rapid detection of amplification products using lateral flow devices containing antibodies specific to the incorporated labels, avoiding the need for fluorescence detection or gel electrophoresis. © 2012.

Publication metadata

Author(s): Tomlinson JA, Ostoja-Starzewska S, Adams IP, Miano DW, Abidrabo P, Kinyua Z, Alicai T, Dickinson MJ, Peters D, Boonham N, Smith J

Publication type: Article

Publication status: Published

Journal: Journal of Virological Methods

Year: 2013

Volume: 191

Issue: 2

Pages: 148-154

Print publication date: 01/08/2013

Online publication date: 20/07/2012

ISSN (print): 0166-0934

ISSN (electronic): 1879-0984

Publisher: Elsevier BV

URL: https://doi.org/10.1016/j.jviromet.2012.07.015

DOI: 10.1016/j.jviromet.2012.07.015

PubMed id: 22820076

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