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The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses

Lookup NU author(s): Professor Neil Boonham

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Abstract

The complete nucleotide sequences of RNA 1 and RNA 2 of the nepovirus potato black ringspot virus (PBRSV) from two different isolates were determined, as well as partial sequences from two additional isolates. RNA1 is 7,579-7,598 nucleotides long and contains one single open reading frame (ORF), which is translated into a large polyprotein with 2,325 amino acids and a molecular weight of 257 kDa. The complete sequence of RNA2 ranges from 3857 to 3918 nt between the different isolates. It encodes a polyprotein of 1079-1082 amino acids with a molecular weight of 120 kDa. Sequence comparison using the Pro-Pol region and CP showed that all four isolates formed two distinct groups, corresponding to potato and arracacha, that were closely related to each other and also to tobacco ringspot virus (TRSV). Comparing our data to those obtained with other nepoviruses, our results confirm that PBRSV belongs to a distinct species and is a member of subgroup A in the genus Nepovirus based on its RNA2 size, genome organization, and nucleotide sequence. © 2013 Crown Copyright.

Publication metadata

Author(s): Souza Richards R, Adams IP, Kreuze JF, De Souza J, Cuellar W, Dullemans AM, van der Vlugt RAA, Glover R, Hany U, Dickinson M, Boonham N

Publication type: Article

Publication status: Published

Journal: Archives of Virology

Year: 2014

Volume: 159

Issue: 4

Pages: 811-815

Print publication date: 01/04/2014

Online publication date: 13/10/2013

Acceptance date: 26/09/2013

ISSN (print): 0304-8608

ISSN (electronic): 1432-8798

Publisher: Springer-Verlag Wien

URL: https://doi.org/10.1007/s00705-013-1871-8

DOI: 10.1007/s00705-013-1871-8

PubMed id: 24122155

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