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Lookup NU author(s): Dr Anetta Svitorka Hartlova, Dr Julien Peltier, Professor Matthias TrostORCiD
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Phagocytosis plays an essential role in the immune system for the defense against invading microorganisms and the clearing of apoptotic cells. After internalization, the newly formed phagosome is constantly remodeled by fusion with early endosomes, late endosomes, and lysosomes. These changes ultimately deliver the engulfed material into the terminal degradative compartments known as phagolysosomes. However, defective phagosome maturation can result in inflammatory or autoimmune disease depending on the type of phagosome cargo. Therefore, characterization of the components involved in phagosome formation and maturation is important for a better understanding of macrophage physiological and pathological functions. In this chapter we describe a step-by-step protocol for the isolation of large-scale latex/polystyrene bead phagosome preparations with high degrees of purity for Western blotting analysis of phagosome maturation.
Author(s): Härtlova A, Peltier J, Bilkei-Gorzo O, Trost M
Editor(s): Roberto Botelho
Publication type: Book Chapter
Publication status: Published
Book Title: Phagocytosis and Phagosomes: Methods and Protocols
Year: 2017
Volume: 1519
Pages: 241-248
Print publication date: 07/04/2017
Online publication date: 05/11/2016
Acceptance date: 17/08/2016
Publisher: Springer
Place Published: New York
URL: https://doi.org/10.1007/978-1-4939-6581-6_16
DOI: 10.1007/978-1-4939-6581-6_16
PubMed id: 27815884
Library holdings: Search Newcastle University Library for this item
ISBN: 9781493965816