Protocols for calculating reaction kinetics and thermodynamics

Dolfing, J

doi:10.1007/8623 2015 109

Protocols for calculating reaction kinetics and thermodynamics

Lookup NU author(s): Dr Jan Dolfing ORCiD

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Abstract

Thermodynamics is central to our understanding of the ecology and physiology of microbial ecosystems. In physiological research, thermodynamics is used to determine the directionality of a reaction or the feasibility of a pathway. In ecological research, thermodynamics is used to determine the feasibility of a process and to rationalize the sequence of redox reactions in both natural environments and engineered biological systems and increasingly also as a basis to describe microbial activities as a function of environmental factors. This protocol provides a detailed annotated description of how to calculate change in Gibbs free energy values and redox potentials and a brief discussion on linking thermodynamics and kinetics for reactions of biological interest.