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Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover

Lookup NU author(s): Professor Gavin RichardsonORCiD

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Abstract

Although it is accepted that the heart has a limited potential to regenerate cardiomyocytes following injury and that low levels of cardiomyocyte turnover occur during normal ageing, quantification of these events remains challenging. This is in part due to the rarity of the process, the contribution of multiple cellular sources to myocardial maintenance and the fact the DNA duplication within cardiomyocytes often leads to a polyploid cardiomyocyte, and only rarely leads to new cardiomyocytes by cellular division. We have therefore established a new nucleoside labelling protocol in order to trace the contribution of progenitor cells and cardiomyocyte division to the neo-cardiomyocyte population.

Publication metadata

Author(s): Richardson GD

Publication type: Article

Publication status: Published

Journal: Journal of Visualized Experiments

Year: 2016

Issue: 111

Online publication date: 23/05/2016

Acceptance date: 04/08/2015

Date deposited: 04/08/2015

ISSN (electronic): 1940-087X

Publisher: Journal of Visualized Experiments

URL: http://dx.doi.org/10.3791/53979

DOI: 10.3791/53979

PubMed id: 27285379

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