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Lookup NU author(s): Dr Venetia BigleyORCiD, Dr Naomi McGovern, Dr Paul Milne, Dr Rachel Dickinson, Sarah Pagan, Professor Muzlifah Haniffa, Professor Matthew CollinORCiD
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Langerin is a C-type lectin expressed at high level by LCs of the epidermis. Langerin is also expressed by CD8(+)/CD103(+) XCR1(+) cross-presenting DCs of mice but is not found on the homologous human CD141(high) XCR1(+) myeloid DC. Here, we show that langerin is expressed at a low level on DCs isolated from dermis, lung, liver, and lymphoid tissue and that langerin(+) DCs are closely related to CD1c(+) myeloid DCs. They are distinguishable from LCs by the level of expression of CD1a, EpCAM, CD11b, CD11c, CD13, and CD33 and are found in tissues and tissue-draining LNs devoid of LCs. They are unrelated to CD141(high) XCR1(+) myeloid DCs, lacking the characteristic expression profile of cross-presenting DCs, conserved between mammalian species. Stem cell transplantation and DC deficiency models confirm that dermal langerin(+) DCs have an independent homeostasis to LCs. Langerin is not expressed by freshly isolated CD1c(+) blood DCs but is rapidly induced on CD1c(+) DCs by serum or TGF-beta via an ALK-3-dependent pathway. These results show that langerin is expressed outside of the LC compartment of humans and highlight a species difference: langerin is expressed by the XCR1(+) "DC1" population of mice but is restricted to the CD1c(+) "DC2" population of humans (homologous to CD11b(+) DCs in the mouse).
Author(s): Bigley V, McGovern N, Milne P, Dickinson R, Pagan S, Cookson S, Haniffa M, Collin M
Publication type: Article
Publication status: Published
Journal: Journal of Leukocyte Biology
Year: 2015
Volume: 97
Issue: 4
Pages: 627-634
Print publication date: 01/04/2015
Online publication date: 16/12/2014
Acceptance date: 16/10/2014
Date deposited: 10/06/2015
ISSN (print): 0741-5400
ISSN (electronic): 1938-3673
Publisher: Federation of American Societies for Experimental Biology
URL: http://dx.doi.org/10.1189/jlb.1HI0714-351R
DOI: 10.1189/jlb.1HI0714-351R
PubMed id: 25516751
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