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Lookup NU author(s): Vera Ulyanova, Professor Colin Harwood
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Extracellular low-molecular weight guanyl-preferring ribonucleases (LMW RNases) of Bacillus sp. comprise a group of hydrolytic enzymes that share highly similar structural and catalytic characteristics with barnase, a ribonuclease from Bacillus amyloliquefaciens, and binase, a ribonuclease from Bacillus intermedius. Although the physical-chemical and catalytic properties of Bacillus guanyl-preferring ribonucleases are very similar, there is considerably more variation in the environmental conditions that lead to the induction of the genes encoding these RNases. Based on structural differences of their genes the guanyl-preferring ribonucleases have been sub-divided into binase-like and barnase-like groups. Here we show the ability of the key regulator of phosphate deficiency response, PhoP, to direct the transcription of the binase-like RNases but not barnase-like RNases. These results, together with our demonstration that binase-like RNases are induced in response to phosphate starvation, allow us to categorise this group of ribonucleases as new members of Bacillus PhoP regulon. In contrast, the barnase-like ribonucleases are relatively insensitive to the phosphate concentration and the environmental conditions that are responsible for their induction, and the regulatory elements involved, are currently unknown. (C) 2014 Elsevier GmbH. All rights reserved.
Author(s): Ulyanova V, Vershinina V, Ilinskaya O, Harwood CR
Publication type: Article
Publication status: Published
Journal: Microbiological Research
Year: 2015
Volume: 170
Pages: 131-138
Print publication date: 01/01/2015
Online publication date: 29/08/2014
Acceptance date: 24/08/2014
ISSN (print): 0944-5013
ISSN (electronic): 1618-0623
Publisher: Elsevier GmbH - Urban und Fischer
URL: http://dx.doi.org/10.1016/j.micres.2014.08.005
DOI: 10.1016/j.micres.2014.08.005
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