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Lookup NU author(s): Dr Ling Juan Wu
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
Plasmid conjugation plays a significant role in the dissemination of antibiotic resistance and pathogenicity determinants. Understanding how conjugation is regulated is important to gain insights into these features. Little is known about regulation of conjugation systems present on plasmids from Gram-positive bacteria. pLS20 is a native conjugative plasmid from the Gram-positive bacterium Bacillus subtilis. Recently the key players that repress and activate pLS20 conjugation have been identified. Here we studied in detail the molecular mechanism regulating the pLS20 conjugation genes using both in vivo and in vitro approaches. Our results show that conjugation is subject to the control of a complex genetic switch where at least three levels of regulation are integrated. The first of the three layers involves overlapping divergent promoters of different strengths regulating expression of the conjugation genes and the key transcriptional regulator Rco(LS20). The second layer involves a triple function of Rco(LS20) being a repressor of the main conjugation promoter and an activator and repressor of its own promoter at low and high concentrations, respectively. The third level of regulation concerns formation of a DNA loop mediated by simultaneous binding of tetrameric Rco(LS20) to two operators, one of which overlaps with the divergent promoters. The combination of these three layers of regulation in the same switch allows the main conjugation promoter to be tightly repressed during conditions unfavorable to conjugation while maintaining the sensitivity to accurately switch on the conjugation genes when appropriate conditions occur. The implications of the regulatory switch and comparison with other genetic switches involving DNA looping are discussed.
Author(s): Ramachandran G, Singh PK, Luque-Ortega JR, Yuste L, Alfonso C, Rojo F, Wu LJ, Meijer WJJ
Publication type: Article
Publication status: Published
Journal: PLOS Genetics
Year: 2014
Volume: 10
Issue: 10
Online publication date: 23/10/2014
Acceptance date: 03/09/2014
Date deposited: 06/03/2015
ISSN (print): 1553-7390
ISSN (electronic): 1553-7404
Publisher: Public Library of Science
URL: http://dx.doi.org/10.1371/journal.pgen.1004733
DOI: 10.1371/journal.pgen.1004733
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