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Quantitative analysis of tyrosine hydroxylase mRNA for sensitive detection of neuroblastoma cells in blood and bone marrow

Lookup NU author(s): Dr Frida Ponthan

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Abstract

BACKGROUND: Sensitive monitoring of minimal residual disease may improve the treatment of neuroblastoma in children. To detect and monitor neuroblastoma cells in blood and bone marrow, we developed a quantitative method for the analysis of tyrosine hydroxylase mRNA. METHODS: We used real-time reverse transcription-PCR. The calibrator was constructed from a segment of tyrosine hydroxylase mRNA that included the target. Blood and bone marrow samples from 24 children with neuroblastoma and 1 child with ganglioneuroma were analyzed. Controls were blood samples from the cords of 40 babies, from 58 children 6 months to 15 years of age, and from 34 healthy adults, as well as from 12 children with other diseases. RESULTS: The detection limit was approximately 70 transcripts/mL. All 144 blood controls were below this limit. At diagnosis, blood tyrosine hydroxylase mRNA was higher in children with widespread disease (stage 4/4S; n = 6; range, 203-46,000 transcripts/mL) than in patients with localized disease (stages 1-3; n = 6;

Publication metadata

Author(s): Träger C, Kogner P, Lindskog M, Ponthan FM, Kullman A, Kågedal B

Publication type: Article

Publication status: Published

Journal: Clinical Chemistry

Year: 2003

Volume: 49

Issue: 1

Pages: 104-112

ISSN (print): 0009-9147

ISSN (electronic): 1530-8561

URL: http://dx.doi.org/10.1373/49.1.104

DOI: 10.1373/49.1.104

Notes: Clinical Trial Journal Article Research Support, Non-U.S. Gov't United States

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