ePrints

Comparison of data analysis parameters and MS/MS fragmentation techniques for quantitative proteome analysis using isobaric peptide termini labeling (IPTL)

Lookup NU author(s): Dr Achim Treumann

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

Isobaric peptide termini labeling (IPTL) is a quantification method which permits relative quantification using quantification points distributed throughout the whole tandem mass spectrometry (MS/MS) spectrum. It is based on the complementary derivatization of peptide termini with different isotopes resulting in isobaric peptides. Here, we use our recently developed software package IsobariQ to investigate how processing and data analysis parameters can improve IPTL data. Deisotoping provided cleaner MS/MS spectra and improved protein identification and quantification. Denoising should be used with caution because it may remove highly regulated ion pairs. An outlier detection algorithm on the ratios within every individual MS/MS spectrum was beneficial in removing false-positive quantification points. MS/MS spectra using IPTL typically contain two peptide series with complementary labels resulting in lower Mascot ion scores than non-labeled equivalent peptides. To avoid this penalty, the two chemical modifications for IPTL were specified as variables including satellite neutral losses of tetradeuterium with positive loss for the heavy isotopes and negative loss for the light isotopes. Thus, the less dominant complementary ion series were not considered for the scoring, which improved the ion scores significantly. In addition, we showed that IPTL was suitable for fragmentation by electron transfer dissociation (ETD) and higher energy collisionally activated dissociation (HCD) besides the already reported collision-induced dissociation (CID). Notably, ETD and HCD data can be identified and quantified using IsobariQ. ETD outperformed CID and HCD only for charge states ≥4+ but yielded in total fewer protein identifications and quantifications. In contrast, the high-resolution information of HCD fragmented peptides provided most identification and quantification results using the same scan speed.

Publication metadata

Author(s): Koehler CJ, Arntzen MØ, Treumann A, Thiede B

Publication type: Article

Publication status: Published

Journal: Analytical and Bioanayltical Chemistry

Year: 2012

Volume: 404

Issue: 4

Pages: 1103-1114

Print publication date: 31/03/2012

ISSN (print): 1618-2642

ISSN (electronic): 1618-2650

Publisher: Springer

URL: http://dx.doi.org/10.1007/s00216-012-5949-z

DOI: 10.1007/s00216-012-5949-z

Altmetrics

Share