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Lookup NU author(s): Dr Naoko Sano
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This study describes the development of a functional porous polymer for use as a scaffold to support 3D hepatocyte culture. A high internal phase emulsion (HIPE) is prepared containing the monomers styrene (STY), divinylbenzene (DVB), and 2-ethylhexyl acrylate (EHA) in the external oil phase and the monomer acrylic acid (Aa) in the internal aqueous phase. Upon thermal polymerization with azobisisobutyronitrile (AIBN), the resulting porous polymer (polyHIPE) is found to have an open-cell morphology and a porosity of 89%, both suitable characteristics for 3D cell scaffold applications. X-ray photoelectron spectroscopy reveals that the polyHIPE surface contained 7.5% carboxylic acid functionality, providing a useful substrate for subsequent surface modifications and bio-conjugations. Initial bio-compatibility assessments with human hepatocytes show that the acid functionality does not have any detrimental effect on cell adhesion. It is therefore believed that this material can be a useful precursor scaffold towards 3D substrates that offer tailored surface functionality for enhanced cell adhesion.
Author(s): Hayward AS, Sano N, Przyborski SA, Cameron NR
Publication type: Article
Publication status: Published
Journal: Macromolecular Rapid Communications
Year: 2013
Volume: 34
Issue: 23-24
Pages: 1844-1849
Print publication date: 14/11/2013
ISSN (print): 1022-1336
ISSN (electronic): 1521-3927
Publisher: Wiley - VCH Verlag GmbH & Co. KGaA
URL: http://dx.doi.org/10.1002/marc.201300709
DOI: 10.1002/marc.201300709
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