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Lookup NU author(s): Professor Hermann Josef Vormoor
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The development of stem-cell gene therapy is hindered by the absence of repopulation assays for primitive human hematopoietic cells. Current methods of gene transfer rely on in vitro colony-forming cell (CFC) and long-term culture-initiating cell (LTC-IC) assays, as well as inference from other mammalian species. We have identified a novel human hematopoietic cell, the SCID-repopulating cell (SRC), a cell more primitive than most LTC-ICs and CFCs. The SRC, exclusively present in the CD4+CD8- fraction, is capable of multilineage repopulation of the bone marrow of nonobese diabetic mice with severe combined immunodeficiency disease (NOD/SCID mice). SRCs were rarely transduced with retroviruses, distinguishing them from most CFCs and LTC-ICs. This observation is consistent with the low level of gene marking seen in human gene therapy trials. An SRC assay may aid in the characterization of hematopoiesis, as well as the improvement of transduction methods.
Author(s): Larochelle A, Vormoor J, Hanenberg H, Wang JCY, Bhatia M, Lapidot T, Moritz T, Murdoch B, Xiao XL, Kato I, Williams DA, Dick JE
Publication type: Article
Publication status: Published
Journal: Nature Medicine
Year: 1996
Volume: 2
Issue: 12
Pages: 1329-1337
Print publication date: 01/12/1996
ISSN (print): 1078-8956
ISSN (electronic): 1546-170X
URL: http://dx.doi.org/10.1038/nm1296-1329
DOI: 10.1038/nm1296-1329
Notes: 1078-8956 (Print) Journal Article
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