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Annexin A9 is a periplakin interacting partner in membrane-targeted cytoskeletal linker protein complexes

Lookup NU author(s): Dr Veronika Boczonadi

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Abstract

Periplakin regulates keratin organisation and participates in the assembly of epidermal cornified envelopes. A proteomic approach identified annexin A9 as a novel interacting partner for periplakin N-terminus. The presence of annexin A9 in complexes with periplakin was confirmed by immunoblotting of proteins immunoprecipitated by anti-HA or anti-annexin A9 antibodies. Both endogenous and GFP-tagged annexin A9 co-localise with endogenous periplakin and transfected periplakin N-terminus at MCF-7 cell borders and aggregate after Okadaic acid treatment. Annexin A9 and periplakin co-localise in the epidermis and annexin A9 is up-regulated in differentiating keratinocytes, but the epidermal annexin A9 expression does not require periplakin.

Publication metadata

Author(s): Boczonadi V, Määttä A

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 2012

Volume: 19

Issue: 586

Pages: 3090-3096

Print publication date: 21/09/2012

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/j.febslet.2012.07.057

DOI: 10.1016/j.febslet.2012.07.057

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