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Lookup NU author(s): Dr Gordon Beakes
The mycoparasitic oomycete Pythium oligandrum is homothallic, producing an abundance of thick-walled spiny oospores in culture. After mining a cDNA sequence dataset, we identified a family of genes that code for small tyrosine rich (Pythium oligandrumsmall tyrosine rich (PoStr)) proteins. Sequence analysis identified similarity between the PoStr proteins and putative glycine-rich cell wall proteins from the related plant pathogenic oomycete Pythium ultimum, and mating-induced genes from the oomycete Phytophthora infestans. Expression analysis showed that PoStr transcripts accumulate during oospore production in culture and immunolocalisation indicates the presence of these proteins in oogonial and oospore cell walls. PoStr protein abundance correlated positively with production of oogonia as determined by antibiotic-mediated oogonia suppression. To further characterise the role of PoStr proteins in P. oligandrum oospore production, we silenced this gene family using homology-dependent gene silencing. This represents the first characterisation of genes using gene silencing in a Pythium species. Oospores from silenced strains displayed major ultrastructural changes and were sensitive to degradative enzyme treatment. Oogonia of silenced strains either appeared to be arrested at the mature oosphere stage of development or in around 40 % of the structures, showed a complete suppression of oospore formation. Suppressed oogonia were highly vacuolated and the oogonium wall was thickened by a new inner wall layer. Our data suggest PoStr proteins are probably integral structural components of the normal oospore cell wall and play a key role in oospore formation.
Author(s): Grenville-Biggs LJ, Horner NR, Phillips AJ, Beakes GW, van West P
Publication type: Article
Publication status: Published
Journal: Fungal Biology
Year: 2013
Volume: 117
Issue: 3
Pages: 163-172
Print publication date: 19/01/2013
Date deposited: 28/03/2013
ISSN (print): 1878-6146
ISSN (electronic): 1878-6162
Publisher: Elsvier
URL: http://dx.doi.org/10.1016/j.funbio.2013.01.001
DOI: 10.1016/j.funbio.2013.01.001
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