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Lookup NU author(s): Naif Alsharif, Dr Mark Birch, Dr Ben Horrocks, Dr Harish Datta
There is a lack of suitable in vitro techniques for assaying osteoclastic (OC) resorptive activity in real-time; the existing assays are cumbersome, insensitive and retrospective. We have developed a sensitive real-time assay by impregnating a hydroxyapatite matrix with alkyl-capped silicon nanocrystals. Resorption of the matrix by OC releases the nanocrystals, which are internalised by the cell and detected as an increase in OC luminescence. Our choice of these nanocrystals was motivated by their pH-independent luminescence, which is proportional to concentration over a wide range, and by their rapid uptake without acute cytotoxicity. In this in vitro assay, OCs are inhibited by calcitonin (CT), methyl-b-cyclodextrin (MCD) and filipin, and stimulated by RANKL in the expected manner. The kinetics of the assay exhibit a lag phase followed by a growth of cell luminescence intensity and the whole time-course is satisfactorily described by the logistic equation.
Author(s): Alsharif NH, Al-Said SAF, Birch MA, Horrocks BR, Datta HK
Publication type: Article
Publication status: Published
Journal: Small
Year: 2013
Volume: 9
Issue: 21
Pages: 3685-3692
Print publication date: 06/05/2013
Date deposited: 28/03/2013
ISSN (print): 1613-6810
ISSN (electronic): 1613-6829
Publisher: Wiley - VCH Verlag GmbH & Co. KGaA
URL: http://dx.doi.org/10.1002/smll.201203184
DOI: 10.1002/smll.201203184
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