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Lookup NU author(s): Professor Maya Sieber-Blum
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EPI-NCSC are remnants of the embryonic neural crest in an adult location, the bulge of hair follicles. They are multipotent stem cells that have the physiological property to generate a wide array of differentiated cell types, including neurons, nerve supporting cells, smooth muscle cells, bone/cartilage cells and melanocytes. EPI-NCSC are easily accessible in the hairy skin and can be isolated as a highly pure population of stem cells. This video provides a detailed protocol for preparing mouse EPI-NCSC cultures from whisker follicles. The whisker pad of an adult mouse is removed, and whisker follicles dissected. The follicles are then cut longitudinally and subsequently transversely above and below the bulge region. The bulge is removed from the collagen capsule and placed in a culture plate. EPI-NCSC start to emigrate from the bulge explants 3 to 4 days later.
Author(s): Sieber-Blum M, Hu YF
Publication type: Article
Publication status: Published
Journal: Journal of Visualized Experiments
Year: 2013
Volume: 15
Print publication date: 09/05/2013
ISSN (electronic): 1940-087X
Publisher: Journal of Visualized Experiments
URL: http://dx.doi.org/10.3791/772
DOI: 10.3791/772
Notes: The video component of this article can be found at: http://www.jove.com/video/772/ video publication: 23,192 views
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