ePrints

Thiol peroxidases mediate specific genome-wide regulation of gene expression in response to hydrogen peroxide

Lookup NU author(s): Dr Julian Rutherford

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

Hydrogen peroxide is thought to regulate cellular processes by direct oxidation of numerous cellular proteins, whereas antioxidants, most notably thiol peroxidases, are thought to reduce peroxides and inhibit H2O2 response. However, thiol peroxidases have also been implicated in activation of transcription factors and signaling. It remains unclear if these enzymes stimulate or inhibit redox regulation and whether this regulation is widespread or limited to a few cellular components. Herein, we found that Saccharomyces cerevisiae cells lacking all eight thiol peroxidases were viable and withstood redox stresses. They transcriptionally responded to various redox treatments, but were unable to activate and repress gene expression in response to H2O2. Further studies involving redox transcription factors suggested that thiol peroxidases are major regulators of global gene expression in response to H2O2. The data suggest that thiol peroxidases sense and transfer oxidative signals to the signaling proteins and regulate transcription, whereas a direct interaction between H2O2 and other cellular proteins plays a secondary role.

Publication metadata

Author(s): Fomenko DE, Koc A, Agisheva N, Jacobsen M, Kaya A, Malinouski M, Rutherford JC, Siu KL, Jin DY, Winge DR, Gladyshev VN

Publication type: Article

Publication status: Published

Journal: Proceedings of the National Academy of Sciences

Year: 2011

Volume: 108

Issue: 7

Pages: 2729-2734

Print publication date: 31/01/2011

ISSN (print): 0027-8424

ISSN (electronic): 1091-6490

Publisher: National Academy of Sciences

URL: http://dx.doi.org/10.1073/pnas.1010721108

DOI: 10.1073/pnas.1010721108

PubMed id: 21282621

Altmetrics

Share