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Yuhushiella deserti gen. nov., sp. nov., a new member of the suborder Pseudonocardineae

Lookup NU author(s): Jian Wang, Zi Zhang, Professor Michael Goodfellow

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Abstract

A thermotolerant, Gram-stain-positive, aerobic, sporangium-forming actinomycete, strain RA45(T), was isolated from a desert region in Xinjiang Uigur Autonomous Region, north-western China. Comparative analysis of the 16S rRNA gene sequence and phenotypic characterization revealed that strain RA45(T) belonged phylogenetically to the family Pseudonocardiaceae of the suborder Pseudonocardineae. Strain RA45(T) showed more than 5% 16S rRNA gene sequence divergence from recognized species of genera in the family Pseudonocardiaceae, forming a distinct lineage within the evolutionary radiation occupied by the genera Amycolatopsis, Prauserella, Thermocrispum, Saccharomonospora, Saccharopolyspora and Sciscionella, but distinct from each of them. The affiliation to the family was supported by the presence of suborder- and family-specific 16S rRNA signature nucleotides, a DNA G + C content of 69.9 mol%, the presence of meso-diaminopimelic acid, ribose, arabinose, glucose and galactose, which are characteristic components of cell-wall chemotype IV of actinomycetes, the presence of menaquinone MK-9(H-4) as the major respiratory lipoquinone, a lack of mycolic acids and the presence of an N-acetylated type of muramic acid. However, strain RA45(T) differed from known genera of the family in its polar lipid composition: the major phospholipids were phosphatidylethanolamine, phosphatidylinositol mannosides, phosphatidylmethylethanolamine, diphosphatidylglycerol, phospholipids of unknown structure and phospholipids of unknown structure containing glucosamine (phospholipid type IV). Based on its morphological, chemotaxonomic and phylogenetic characteristics, strain RA45(T) is considered to represent a novel species of a new genus in the family Pseudonocardiaceae, for which the name Yuhushiella deserti gen. nov., sp. nov. is proposed. The type strain of Yuhushiella deserti is RA45(T) (=CGMCC 4.5579(T) =JCM 16584(T)).


Publication metadata

Author(s): Mao J, Wang JA, Dai HQ, Zhang ZD, Tang QY, Ren BA, Yang N, Goodfellow M, Zhang LX, Liu ZH

Publication type: Article

Publication status: Published

Journal: International Journal of Systematic and Evolutionary Microbiology

Year: 2011

Volume: 61

Pages: 621-630

Print publication date: 01/03/2011

ISSN (print): 1466-5026

ISSN (electronic): 1070-6259

Publisher: Society for General Microbiology

URL: http://dx.doi.org/10.1099/ijs.0.019588-0

DOI: 10.1099/ijs.0.019588-0


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Funding

Funder referenceFunder name
Hundred Talents Program
062A131BB4Chinese Academy of Sciences
2006AA09Z402National 863 Project
2007AA09Z443National 863 Project
200803034Chinese Ministry of Agriculture
30700015National Natural Science Foundation of China
2007AA021300Hi-Tech Research and Development Program of China

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