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Single-sweep voltage sensitive dye imaging of interacting identified neurons

Lookup NU author(s): Dr Wolfgang Stein, Dr Peter Andras

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Abstract

The simultaneous recording of many individual neurons is fundamental to understanding the integral functionality of neural systems. Imaging with voltage-sensitive dyes (VSDs) is a key approach to achieve this goal and a promising technique to supplement electrophysiological recordings. However, the lack of connectivity maps between imaged neurons and the requirement of averaging over repeated trials impede functional interpretations. Here we demonstrate fast, high resolution and single-sweep VSD imaging of identified and synaptically interacting neurons. We show for the first time the optical recording of individual action potentials and mutual inhibitory synaptic input of two key players in the well-characterized pyloric central pattern generator in the crab stomatogastric ganglion (STG). We also demonstrate the presence of individual synaptic potentials from other identified circuit neurons. We argue that imaging of neural networks with identifiable neurons with well-known connectivity, like in the STG, is crucial for the understanding of emergence of network functionality.

Publication metadata

Author(s): Stein W, Städele C, Andras P

Publication type: Article

Publication status: Published

Journal: Journal of Neuroscience Methods

Year: 2011

Volume: 194

Issue: 2

Pages: 224-234

Print publication date: 20/10/2010

ISSN (print): 0165-0270

ISSN (electronic): 1872-678X

Publisher: Elsevier BV

URL: http://dx.doi.org/10.1016/j.jneumeth.2010.10.007

DOI: 10.1016/j.jneumeth.2010.10.007

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