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Control of signaling in a MAP-kinase pathway by an RNA-binding protein

Lookup NU author(s): Christine Aldridge

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Abstract

Signaling-protein mRNAs tend to have long untranslated regions (UTRs) containing binding sites for RNA-binding proteins regulating gene expression. Here we show that a PUF-family RNA-binding protein, Mpt5, represses the yeast MAP-kinase pathway controlling differentiation to the filamentous form. Mpt5 represses the protein levels of two pathway components, the Ste7 MAP-kinase kinase and the Tec1 transcriptional activator, and negatively regulates the kinase activity of the Kss1 MAP kinase. Moreover, Mpt5 specifically inhibits the output of the pathway in the absence of stimuli, and thereby prevents inappropriate cell differentiation. The results provide an example of what may be a genome-scale level of regulation at the interface of signaling networks and protein-RNA binding networks. © 2007 Prinz et al.

Publication metadata

Author(s): Prinz S, Aldridge C, Ramsey SA, Taylor RJ, Galitski T

Publication type: Article

Publication status: Published

Journal: PLoS ONE

Year: 2007

Volume: 2

Issue: 2

Date deposited: 07/11/2011

ISSN (print): 1932-6203

ISSN (electronic):

Publisher: Public Library of Science

URL: http://dx.doi.org/10.1371/journal.pone.0000249

DOI: 10.1371/journal.pone.0000249

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