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Lookup NU author(s): Dr Simon Wilkinson
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Skin barrier function is a key parameter to consider when performing in vitro percutaneous absorption studies. Whilst tritiated water flux measurements were often used to assess skin integrity, recent decades have witnessed the emergence of the more rapid and user-friendly transepidermal water loss (TEWL) approach. Yet to date, the nature of the correlation between TEWL and skin barrier function in vitro has still not been comprehensively established. In this study, a novel TEWL device, operating on a cold-induced vapour sink principle, was used to probe the barrier function of full-thickness porcine skin. The method was sufficiently sensitive to show the influence of punctures on barrier function although the observed non-linear pattern suggested tissue swelling processes and/or capillary action could be occurring. The results of various surfactant application experiments strongly suggested that TEWL was indeed largely predictive of skin sample integrity. A key finding was that basal TEWL was linearly correlated with basal tritiated water flux (r(2) = 0.80, n = 63). Thus, a dedicated TEWL method can be used as a good alternative to water flux measurements for assessing full-thickness skin barrier function. (C) 2009 Elsevier Ltd. All rights reserved.
Author(s): Elmahjoubi E, Frum Y, Eccleston GM, Wilkinson SC, Meidan VM
Publication type: Article
Publication status: Published
Journal: Toxicology in Vitro
Year: 2009
Volume: 23
Issue: 7
Pages: 1429-1435
ISSN (print): 0887-2333
ISSN (electronic): 1879-3177
Publisher: Pergamon
URL: .thtp://dx.doi.org/10.1016/j.tiv.2009.06.030
DOI: 10.1016/j.tiv.2009.06.030
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