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Recruitment of Condensin to Replication Origin Regions by ParB/SpoOJ Promotes Chromosome Segregation in B. subtilis

Lookup NU author(s): Dr Stephan Gruber, Professor Jeff ErringtonORCiD

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Abstract

Proper segregation of DNA replication products is essential in all cells. In Bacillus subtilis, two protein complexes have been implicated in this process: the ParAB homologs, Soj and SpoOJ, and the bacterial Smc/ScpAB complex, also called condensin. Here we demonstrate that Smc is highly enriched in the region around the origin of replication, specifically near parS sites to which SpoOJ binds and at highly transcribed genes. Furthermore, we find that efficient recruitment of Smc to a large region around the origin of replication depends on the presence of SpoOJ. We show that SpoOJ performs two independent functions: regulation of initiation of DNA replication via Soj and promotion of chromosome segregation by Smc recruitment. Our results demonstrate a direct functional interaction between two widely conserved systems involved in chromosome replication and segregation.

Publication metadata

Author(s): Gruber S, Errington J

Publication type: Article

Publication status: Published

Journal: Cell

Year: 2009

Volume: 137

Issue: 4

Pages: 685-696

ISSN (print): 0092-8674

ISSN (electronic): 1097-4172

Publisher: Cell Press

URL: http://dx.doi.org/10.1016/j.cell.2009.02.035

DOI: 10.1016/j.cell.2009.02.035

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