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Bacteria of the sulfur cycle in the sediments of gold mine tailings, Kuznetsk Basin, Russia

Lookup NU author(s): David Banks

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Abstract

The number and diversity of culturable microorganisms involved in sulfur oxidation and sulfate reduction were investigated in the oxidized sediments of gold mine tailings, Kuznetsk Basin, Russia. The sediments had a low pH (2.4-2.8), high SO (4) (2-) content (up to 22 g/l), and high concentrations of dissolved metals. The arsenic content was as high as 1.9 g/l. Bacterial phylogeny in microcosms was investigated by amplification of 16S rRNA gene fragments with subsequent denaturing gradient gel electrophoresis (DGGE). Spore-forming bacteria Desulfosporosinus were the only bacteria revealed for which the capacity for dissimilatory sulfate reduction is known. Strain Desulfosporosinus sp. DB was obtained in pure culture, and it was phylogenetically remote from other cultured and uncultured members of the genus. No sulfate-reducing members of the Deltaproteobacteria were detected. The Firmicutes members were the most numerous phylotypes in the microcosms, including a separate cluster with the similarity to Pelotomaculum not exceeding 94%. Acidithiobacillus ferrooxidans and A. caldus were found in anaerobic and microaerophilic microcosms. The number of sulfate reducers did not exceed 9.5 x 10(2) cells/ml.

Publication metadata

Author(s): Karnachuk OV, Gerasimchuk AL, Banks D, Frengstad B, Stykon GA, Tikhonova ZL, Kaksonen A, Puhakka J, Yanenko AS, Pimenov NV

Publication type: Article

Publication status: Published

Journal: Microbiology

Year: 2009

Volume: 78

Issue: 4

Pages: 483-491

ISSN (print): 0026-2617

ISSN (electronic): 1608-3237

Publisher: MAIK Nauka/Interperiodica

URL: http://dx.doi.org/10.1134/S0026261709040122

DOI: 10.1134/S0026261709040122

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