Development of Specific Oligonucleotide Probes to Detect Vibrio Species A Preliminary Study

Molini, U; Thanantong, N; Giangaspero, A; Sparagano, OAE

doi:10.1196/annals.1428.049

Development of Specific Oligonucleotide Probes to Detect Vibrio Species A Preliminary Study

Lookup NU author(s): Umberto Molini, Narut Thanantong, Dr Olivier Sparagano

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Abstract

Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.