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Dynamic analysis of STAT6 signalling in living cells

Lookup NU author(s): Dr Glyn NelsonORCiD

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Abstract

Functional activity of N- and C-terminal fluorescent fusion proteins between STAT6 and EGFP was demonstrated through IL-4-dependent transcriptional activation and nuclear translocation. The N-terminal (EGFP-STAT6) fusion protein appeared to be more active than the C-terminal fusion. In HEK-293 cells both fusion proteins formed fluorescent nuclear foci following IL-4 stimulation, but in HeLa cells nuclear accumulation was homogeneous. Stimulation of the NF-kappaB pathway through TNFalpha treatment, or expression of p65-EGFP fusion protein, repressed both basal STAT6-dependent transcriptional activity and the extent of activation in response to IL-4. This indicates a novel mechanism of inhibition of STAT6 signalling by NF-kappaB activation. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

Publication metadata

Author(s): Nelson G, Wilde GJC, Spiller DG, Sullivan E, Unitt JF, White MRH

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 2002

Volume: 532

Issue: 1-2

Pages: 188-192

Print publication date: 04/12/2002

Online publication date: 09/11/2002

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468

Publisher: John Wiley & Sons Ltd

URL: https://doi.org/10.1016/S0014-5793(02)03672-4

DOI: 10.1016/S0014-5793(02)03672-4

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