Browse by author
Lookup NU author(s): Claire Taylor, Professor Andrew Pearson, Professor Archibald Malcolm
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Aims: To detect the presence of human herpes virus 6 (HHV6) and endogenous biotin in paraffin wax embedded and frozen salivary glands. Methods: Two stage indirect and streptavidin-biotin immunoperoxidase techniques were used to visualise the antigens. Results: HHV6 could not be shown in any of the tissues. However, considerable endogenous biotin antigenicity was detected in the glandular elements of the paraffin wax embedded material. Conclusions: Results obtained with avidin-biotin detection systems should be interpreted with caution, especially when glandular epithelium is being stained. This may apply to both immunoperoxidase and in situ hybridisation techniques. The use of an anti-biotin antibody as a standard control should be considered.
Author(s): Green M, Sviland L, Taylor CE, Peiris M, McCarthy AL, Pearson ADJ, Malcolm AJ
Publication type: Article
Publication status: Published
Journal: Journal of Clinical Pathology
Year: 1992
Volume: 45
Issue: 9
Pages: 788-790
Print publication date: 01/09/1992
ISSN (print): 0021-9746
ISSN (electronic): 1472-4146
URL: http://dx.doi.org/10.1136/jcp.45.9.788
DOI: 10.1136/jcp.45.9.788
PubMed id: 1328329
Altmetrics provided by Altmetric
