Toggle Main Menu Toggle Search

Open Access padlockePrints

Primary hepatocellular carcinoma localised by a radiolabelled monoclonal antibody

Lookup NU author(s): Emeritus Professor Oliver James, Professor Nicola CurtinORCiD, Professor Margaret Bassendine

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

A rat monoclonal antibody, YPC2/38.8, was selected from a panel of antibodies derived by immunising rats with fresh human colorectal carcinoma. It was found to bind to a 30 000 dalton protein present on the cell surface of normal colon and liver. This protein was increased 10-fold on primary hepatocellular carcinoma (PHC) cells. After labelling with 131I, YPC2/38.8 was shown to localise human PHCs grown as xenografts in immunosuppressed mice. Sixteen patients with PHC were given 1 mg of purified antibody labelled with 1 mCi of 131I by slow intravenous injection. In 8 out of 9 patients with PHC arising in non-cirrhotic livers, good tumour images were obtained on gamma camera or rectilinear scans, but in 7 patients who had developed PHC on the background of established hepatic cirrhosis, no tumour images were seen. Subsequent studies revealed that the 30K antigen recognised by this antibody was present in increased quantity on PHC cells and the regenerating liver cells in cirrhosis. We conclude that YPC2/38.8 may have potential for diagnostic localisation and possibly thence for the selective targeting of drugs or toxins in patients with PHC arising in a liver unaffected by significant parenchymal disease.


Publication metadata

Author(s): Markham N, Ritson A, James OFW, Curtin NJ, Bassendine MF, Sikora K

Publication type: Article

Publication status: Published

Journal: Journal of Hepatology

Year: 1986

Volume: 2

Issue: 1

Pages: 25-31

ISSN (print): 0168-8278

ISSN (electronic): 1600-0641

URL: http://dx.doi.org/10.1016/S0168-8278(86)80005-8

DOI: 10.1016/S0168-8278(86)80005-8

PubMed id: 3005388


Altmetrics

Altmetrics provided by Altmetric


Share